Hotspot

psbT-psbN

LSC · 75073-75132 · 60 bp

Back to Chamaelirium

Pi 0.117

MarkerSeek score 74.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 7
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCTAAGGTTCCGACTAAAAA	TTGAAGAACACGGGGACTAG	40.0	50.0	52.5	56.9	110 bp	1.0	45.1
psbT-psbN_p2	CCTAAGGTTCCGACTAAAAA	ATTTGAAGAACACGGGGACT	40.0	45.0	52.5	57.0	112 bp	1.0	45.0
psbT-psbN_p3	CCTAAGGTTCCGACTAAAAA	CATTTGAAGAACACGGGGAC	40.0	50.0	52.5	57.0	113 bp	1.0	45.0
psbT-psbN_p4	CCTAAGGTTCCGACTAAAAA	CCATTTGAAGAACACGGGGA	40.0	50.0	52.5	58.1	114 bp	1.0	44.9
psbT-psbN_p5	CCTAAGGTTCCGACTAAAAA	TCCATTTGAAGAACACGGGG	40.0	50.0	52.5	58.1	115 bp	1.0	44.9