Hotspot

petA-psbJ

LSC · 63499-64604 · 1106 bp

Back to Chamaelirium

Pi 0.0272

MarkerSeek score 67.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 30
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.4	58.3	1234-1236 bp	1.0	75.2
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.4	58.3	1235-1237 bp	1.0	75.2
petA-psbJ_p3	TGCTCTTCTTGGCATCTGTT	GGTTGGGCTCATCTCTGTAG	45.0	55.0	57.4	57.4	1221-1223 bp	1.0	72.8
petA-psbJ_p4	TTGCTCTTCTTGGCATCTGT	GGTTGGGCTCATCTCTGTAG	45.0	55.0	57.4	57.4	1222-1224 bp	1.0	72.8
petA-psbJ_p5	TGCTCTTCTTGGCATCTGTT	CGGTTCATATTCCGGGTTGG	45.0	55.0	57.4	58.7	1235-1237 bp	1.0	72.1