Hotspot

ndhC-trnV-UAC

LSC · 51003-52043 · 1041 bp

Back to Chamaelirium

Pi 0.0189

MarkerSeek score 72.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 19
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATAGGCGCTAAAACTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1149-1176 bp	1.0	81.7
ndhC-trnV-UAC_p2	GGTCCTTCAGTAATAGGCGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.1	57.8	1160-1187 bp	1.0	81.0
ndhC-trnV-UAC_p3	CGGTCCTTCAGTAATAGGCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	1161-1188 bp	1.0	81.0
ndhC-trnV-UAC_p4	AATAGGCGCTAAAACTCCGG	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	1231-1258 bp	1.0	79.3
ndhC-trnV-UAC_p5	AATAGGCGCTAAAACTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	1148-1175 bp	1.0	79.0