Pi
0.0245
MarkerSeek score
63.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 869-875 bp | 1.0 | 81.0 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TTTCCATGCCAAGGCTCAAT | 55.0 | 45.0 | 57.9 | 58.1 | 910-916 bp | 1.0 | 80.5 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 877-883 bp | 1.0 | 80.5 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 901-907 bp | 1.0 | 80.1 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | ACTTAGCAGGTTTCCATGCC | 55.0 | 50.0 | 57.9 | 58.2 | 920-926 bp | 1.0 | 79.6 |