Hotspot

trnK-UUU-rps16

LSC · 4047-4765 · 719 bp

Back to Chamaelirium

Pi 0.0261

MarkerSeek score 67.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 17
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	TTTTCTTGAAAACGGCGCTC	50.0	45.0	58.3	57.9	782-850 bp	1.0	82.5
trnK-UUU-rps16_p2	CATAGCCGCACTTAAAAGCC	TTTTCTTGAAAACGGCGCTC	50.0	45.0	57.5	57.9	787-855 bp	1.0	80.9
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	GCGCTCAACCTACAGTAACT	50.0	50.0	58.3	57.6	768-836 bp	1.0	80.5
trnK-UUU-rps16_p4	TGTGAAAGATCATAGCCGCA	TTTTCTTGAAAACGGCGCTC	45.0	45.0	57.3	57.9	797-865 bp	1.0	79.2
trnK-UUU-rps16_p5	CATAGCCGCACTTAAAAGCC	GCGCTCAACCTACAGTAACT	50.0	50.0	57.5	57.6	773-841 bp	1.0	79.0