Hotspot

rpoC2

LSC · 13364-14938 · 1575 bp

Back to Chamaegastrodia

Pi 0.0468

MarkerSeek score 71.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 1575
variable_sites: 73
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GAGCCCTTATTGCTGCTGAT	GCCTGGGTCAGTGATTTGAA	50.0	50.0	58.0	58.1	2452 bp	0.5	59.1
rpoC2_p2	TGACTTCGAGCCCTTATTGC	GCCTGGGTCAGTGATTTGAA	50.0	50.0	58.0	58.1	2459 bp	0.5	59.1
rpoC2_p3	GAGCCCTTATTGCTGCTGAT	GATGCATTTCCTCTAGCCCC	50.0	55.0	58.0	58.1	2924 bp	0.5	59.1
rpoC2_p4	ACTTCGAGCCCTTATTGCTG	GCCTGGGTCAGTGATTTGAA	50.0	50.0	58.0	58.1	2457 bp	0.5	59.0
rpoC2_p5	TGACTTCGAGCCCTTATTGC	GATGCATTTCCTCTAGCCCC	50.0	55.0	58.0	58.1	2931 bp	0.5	59.0