Hotspot

psbB

LSC · 47369-48449 · 1081 bp

Back to Chamaegastrodia

Pi 0.187

MarkerSeek score 82.00

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1081
variable_sites: 195
pi: 0.187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	GGAATCATTGTGCTTCACGC	CGTGACCGGACTTCTTGAAT	50.0	50.0	58.1	57.9	1776-2780 bp	1.0	87.4
psbB_p2	GGAATCATTGTGCTTCACGC	GTGCCTAGAATTACCACGGG	50.0	55.0	58.1	58.1	2076 bp	0.5	58.8
psbB_p3	CAGGAAGCCGAAAAGGAACT	GTGCCTAGAATTACCACGGG	50.0	55.0	58.1	58.1	1896 bp	0.5	58.8
psbB_p4	GGAATCATTGTGCTTCACGC	AAAGAGCTATTGCGGTACCG	50.0	50.0	58.1	58.1	2359 bp	0.5	58.7
psbB_p5	CAGGAAGCCGAAAAGGAACT	AAAGAGCTATTGCGGTACCG	50.0	50.0	58.1	58.1	2179 bp	0.5	58.7