Hotspot

psaI

LSC · 39206-39310 · 105 bp

Back to Chamaegastrodia

Pi 0.0645

MarkerSeek score 70.50

Primer available yes

Label psaI

Feature Metadata

label_name: psaI
feature_type: gene
region: LSC
length_bp: 105
variable_sites: 6
pi: 0.0645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI_p1	ACCGAGAACCCCTTTTTAGC	AATCGGTCATAACCACTGCC	50.0	50.0	57.8	58.0	1005 bp	0.5	58.1
psaI_p2	ACCGAGAACCCCTTTTTAGC	CGGTCATAACCACTGCCTAC	50.0	55.0	57.8	58.1	1002 bp	0.5	57.9
psaI_p3	ACCGAGAACCCCTTTTTAGC	AATAGACCCGCGATCCCATA	50.0	50.0	57.8	58.1	945 bp	0.5	57.9
psaI_p4	ACCGAGAACCCCTTTTTAGC	TCGGTCATAACCACTGCCTA	50.0	50.0	57.8	58.1	1003 bp	0.5	57.3
psaI_p5	ACCGAGAACCCCTTTTTAGC	GGCCAATTCAGCAGCTTTTT	50.0	45.0	57.8	57.8	1033 bp	0.5	57.0