Hotspot

ndhD-rpl32

SSC · 83146-83515 · 370 bp

Back to Chamaegastrodia

Pi 0.196

MarkerSeek score 69.30

Primer available yes

Label ndhD-rpl32

Feature Metadata

label_name: ndhD-rpl32
feature_type: igs
region: SSC
length_bp: 370
variable_sites: 44
pi: 0.196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-rpl32_p1	TAAATGCACATTCCCCGCTT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.2	58.0	1126 bp	0.5	58.5
ndhD-rpl32_p2	GGCACGAGGATTTGAAGGAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.2	58.0	796 bp	0.5	58.3
ndhD-rpl32_p3	AGTGGCACGAGGATTTGAAG	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.2	58.0	799 bp	0.5	58.2
ndhD-rpl32_p4	GAGTGGCACGAGGATTTGAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.2	58.0	800 bp	0.5	58.2
ndhD-rpl32_p5	TAAATGCACATTCCCCGCTT	AATCTGTTTCCACCGGACAG	45.0	50.0	58.2	57.8	1134 bp	0.5	57.3