Hotspot

psbA

LSC · 127-1188 · 1062 bp

Back to Chamaedorea

Pi 0.0126

MarkerSeek score 48.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 32
pi: 0.0126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGCTCCATTTCTTCAACGA	TGGGAGTCCTTGAAAATTGA	40.0	40.0	55.6	54.4	1144-1149 bp	1.0	42.2
psbA_p2	TTGCTCCATTTCTTCAACGA	TGGGAGTCCTTGAAAATTGAA	40.0	38.1	55.6	55.3	1144-1149 bp	1.0	42.2
psbA_p3	TTGCTCCATTTCTTCAACGA	TTGGGAGTCCTTGAAAATTGA	40.0	38.1	55.6	55.3	1145-1150 bp	1.0	42.2
psbA_p4	TGCTCCATTTCTTCAACGAT	TGGGAGTCCTTGAAAATTGA	40.0	40.0	55.4	54.4	1143-1148 bp	1.0	42.2
psbA_p5	GCTCCATTTCTTCAACGATTCA	TGGGAGTCCTTGAAAATTGA	40.9	40.0	57.3	54.4	1142-1147 bp	1.0	42.2