Hotspot

ycf4-cemA

LSC · 61812-62035 · 224 bp

Back to Chamaedorea

Pi 0.0286

MarkerSeek score 50.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 13
pi: 0.0286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGAAATCAGAGGCCAAGGAG	GGATTTCCCGGTATTCCACC	55.0	55.0	58.0	58.0	459-467 bp	1.0	83.6
ycf4-cemA_p2	CGCATCTTCCTTCGCTTACT	GGATTTCCCGGTATTCCACC	50.0	55.0	58.1	58.0	548-556 bp	1.0	82.7
ycf4-cemA_p3	GTCGCATCTTCCTTCGCTTA	GGATTTCCCGGTATTCCACC	50.0	55.0	58.1	58.0	550-558 bp	1.0	82.7
ycf4-cemA_p4	TCGCATCTTCCTTCGCTTAC	GGATTTCCCGGTATTCCACC	50.0	55.0	58.1	58.0	549-557 bp	1.0	82.7
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTCG	GGATTTCCCGGTATTCCACC	45.0	55.0	57.9	58.0	390-395 bp	0.6	65.2