Hotspot

accD-psaI

LSC · 59801-60785 · 985 bp

Back to Chamaedorea

Pi 0.024

MarkerSeek score 58.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 39
pi: 0.024
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	918-1098 bp	0.8	78.2
accD-psaI_p2	GACAGTACCCGATGGTTCAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1029-1209 bp	0.8	78.1
accD-psaI_p3	GACAGTACCCGATGGTTCAC	TCCCATCGGATCTAGGCAAT	55.0	50.0	58.0	58.0	1081-1261 bp	0.8	78.0
accD-psaI_p4	TGGGATCTGTAGTAGGCGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1348-1528 bp	0.8	77.6
accD-psaI_p5	TGGGATCTGTAGTAGGCGAG	TCCCATCGGATCTAGGCAAT	55.0	50.0	58.0	58.0	1400-1580 bp	0.8	77.5