Hotspot

petN-psbM

LSC · 29798-30496 · 699 bp

Back to Chamaedorea

Pi 0.0115

MarkerSeek score 62.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 15
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	854-867 bp	1.0	71.1
petN-psbM_p2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	57.7	57.6	849-862 bp	1.0	70.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	799-812 bp	1.0	70.0
petN-psbM_p4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.6	57.6	858-871 bp	1.0	68.1
petN-psbM_p5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.6	57.6	857-870 bp	1.0	67.4