Hotspot

trnS-GCU-trnG-UCC

LSC · 8146-9428 · 1283 bp

Back to Chamaedorea

Pi 0.0178
MarkerSeek score 57.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1283
variable_sites
40
pi
0.0178
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCACCGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 256-1412 bp 1.0 57.7
trnS-GCU-trnG-UCC_p2 AATCCACCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 255-1411 bp 1.0 57.7
trnS-GCU-trnG-UCC_p3 CTCGTACAGCGGATTAGCAA ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 273-1429 bp 1.0 56.9
trnS-GCU-trnG-UCC_p4 AACTCGTACAGCGGATTAGC ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 275-1431 bp 1.0 56.9
trnS-GCU-trnG-UCC_p5 AGCAATCCACCGCTTTAGTC ACGAATCACACTTTTACCACT 50.0 38.1 58.3 55.5 258-1414 bp 1.0 56.5