Hotspot

psbK-psbI

LSC · 7413-7808 · 396 bp

Back to Chamaedorea

Pi 0.0411

MarkerSeek score 72.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 30
pi: 0.0411
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	519-524 bp	1.0	83.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	516-521 bp	1.0	81.3
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	517-522 bp	1.0	81.3
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	518-523 bp	1.0	81.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGCCCTGGATCATT	50.0	50.0	57.9	57.4	518-523 bp	1.0	80.8