Hotspot

trnQ-UUG-psbK

LSC · 6898-7226 · 329 bp

Back to Chamaedorea

Pi 0.0164
MarkerSeek score 45.50
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
329
variable_sites
11
pi
0.0164
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CCAAAACCCGCTGCCTTA AGACAGAATTAAGACAGATGCAGA 55.6 37.5 57.9 57.7 411-417 bp 1.0 42.3
trnQ-UUG-psbK_p2 AAAACCCGCTGCCTTACC AGACAGAATTAAGACAGATGCAGA 55.6 37.5 57.9 57.7 409-415 bp 1.0 42.3
trnQ-UUG-psbK_p3 CAAAACCCGCTGCCTTACC AGACAGAATTAAGACAGATGCAGA 57.9 37.5 59.4 57.7 410-416 bp 1.0 42.3
trnQ-UUG-psbK_p4 CCAAAACCCGCTGCCTTAC AGACAGAATTAAGACAGATGCAGA 57.9 37.5 59.4 57.7 411-417 bp 1.0 42.3
trnQ-UUG-psbK_p5 CCAAAACCCGCTGCCTTA GACAGAATTAAGACAGATGCAGA 55.6 39.1 57.9 56.4 410-416 bp 1.0 42.3