Hotspot

ycf1

Genome · 116163-123140 · 6978 bp

Back to Chamaecyparis

Pi 0.0271

MarkerSeek score 57.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 6978
variable_sites: 372
pi: 0.0271
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATCTCTTCCTTCTGACCGC	GGCGGCTTGGTATTGTTCTA	55.0	50.0	58.1	58.0	892-964 bp	1.0	87.2
ycf1_p2	GTTGCTGGACGAAATGAAGC	GGCGGCTTGGTATTGTTCTA	50.0	50.0	58.0	58.0	962-1034 bp	1.0	87.0
ycf1_p3	GTTGCTGGACGAAATGAAGC	GACTTGGGTCGAACCACTTT	50.0	50.0	58.0	58.0	1391-1466 bp	1.0	86.0
ycf1_p4	CTCCCCGTACAATGAGCTTC	GGCGGCTTGGTATTGTTCTA	55.0	50.0	58.1	58.0	873 bp	0.25	49.3
ycf1_p5	CTCCCCGTACAATGAGCTTC	GACTTGGGTCGAACCACTTT	55.0	50.0	58.1	58.0	1302 bp	0.25	49.2