Hotspot

ndhC-trnV-UAC

Genome · 106395-107069 · 675 bp

Back to Chamaecyparis

Pi 0.0251
MarkerSeek score 61.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
675
variable_sites
33
pi
0.0251
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGAGAATGCCAGAATCGGAA GAGAAGGTCTACGGTTCGAG 45.0 55.0 56.9 57.2 775-787 bp 1.0 70.9
ndhC-trnV-UAC_p2 TTGAGAATGCCAGAATCGGA GAGAAGGTCTACGGTTCGAG 45.0 55.0 56.9 57.2 776-788 bp 1.0 70.9
ndhC-trnV-UAC_p3 TGAGAATGCCAGAATCGGAA CGAGAAGGTCTACGGTTCGA 45.0 55.0 56.9 58.9 776-788 bp 1.0 70.2
ndhC-trnV-UAC_p4 TTGAGAATGCCAGAATCGGA CGAGAAGGTCTACGGTTCGA 45.0 55.0 56.9 58.9 777-789 bp 1.0 70.1
ndhC-trnV-UAC_p5 TTGAGAATGCCAGAATCGGAA GAGAAGGTCTACGGTTCGAG 42.9 55.0 57.7 57.2 776-788 bp 1.0 68.7