Hotspot

rps12-clpP

Genome · 101458-102566 · 1109 bp

Back to Chamaecyparis

Pi 0.056

MarkerSeek score 90.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 1109
variable_sites: 104
pi: 0.056
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 90.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGCACATACTCCTTTACGCT	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1178-1291 bp	1.0	87.6
rps12-clpP_p2	TCGCACATACTCCTTTACGC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1179-1292 bp	1.0	87.5
rps12-clpP_p3	GCTGTCTCGCGGTTCTAATA	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	57.5	58.3	1113-1226 bp	1.0	84.7
rps12-clpP_p4	CACATACACCCTCGCACATA	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	57.4	58.3	1190-1303 bp	1.0	83.4
rps12-clpP_p5	ACATACACCCTCGCACATAC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	57.4	58.3	1189-1302 bp	1.0	83.4