Hotspot

rpl16

LSC · 80920-82359 · 1440 bp

Back to Chaetoseris

Pi 0.000602

MarkerSeek score 43.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 2
pi: 0.000602
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	52.6	29.2	59.3	56.2	1602-1603 bp	1.0	40.1
rpl16_p2	CCCGCACCCGCTATTTTT	TCGTTTCTTCTTTTTCTTTTGTGT	55.6	29.2	58.4	56.2	1593-1594 bp	1.0	40.1
rpl16_p3	AAAAAGAAACCCGCACCCG	CGTTTCTTCTTTTTCTTTTGTGTTCA	52.6	30.8	59.3	58.0	1601-1602 bp	1.0	40.1
rpl16_p4	AAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	55.6	29.2	58.6	56.2	1601-1602 bp	1.0	40.1
rpl16_p5	CCCGCACCCGCTATTTTT	CGTTTCTTCTTTTTCTTTTGTGTTCA	55.6	30.8	58.4	58.0	1592-1593 bp	1.0	40.1