Hotspot

matK

LSC · 2064-3584 · 1521 bp

Back to Chaetoseris

Pi 0.000394

MarkerSeek score 40.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 1
pi: 0.000394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCCTTACTACATACTACATCAACA	ACCCCCAAAATCCTCTACCT	33.3	50.0	54.6	57.6	1681 bp	1.0	40.0
matK_p2	ACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	30.4	50.0	53.5	57.6	1673 bp	1.0	40.0
matK_p3	ACATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	29.2	50.0	54.3	57.6	1673 bp	1.0	40.0
matK_p4	ACTACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	30.8	50.0	56.2	57.6	1676 bp	1.0	40.0
matK_p5	CCTTACTACATACTACATCAACA	ACCCCCAAAATCCTCTACCT	34.8	50.0	53.1	57.6	1680 bp	1.0	40.0