Hotspot

psbE-petL

LSC · 64402-65652 · 1251 bp

Back to Chaetoseris

Pi 0.00123

MarkerSeek score 44.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1251
variable_sites: 3
pi: 0.00123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.9	58.2	1398 bp	1.0	78.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.9	58.2	1396 bp	1.0	78.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.8	58.2	1321 bp	1.0	77.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.8	58.2	1319 bp	1.0	77.2
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	45.0	50.0	58.2	58.2	1407 bp	1.0	77.1