Hotspot

accD-psaI

LSC · 58362-59088 · 727 bp

Back to Chaetoseris

Pi 0.000459

MarkerSeek score 43.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 1
pi: 0.000459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGGGTGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.9	58.0	865 bp	1.0	79.7
accD-psaI_p2	AAAAGCGTTCTGGGTGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.9	58.0	864 bp	1.0	79.7
accD-psaI_p3	GCGGCCGAGTATTTATTCCA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.1	58.0	924 bp	1.0	79.1
accD-psaI_p4	AAAAAGCGTTCTGGGTGAGT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.9	58.3	855 bp	1.0	77.6
accD-psaI_p5	AAAAGCGTTCTGGGTGAGTT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.9	58.3	854 bp	1.0	77.6