Hotspot

rpoBb

Genome · 91023-93254 · 2232 bp

Back to Chaetophoropsis

Pi 0.161

MarkerSeek score 82.20

Primer available yes

Label rpoBb

Feature Metadata

label_name: rpoBb
feature_type: gene
region: Genome
length_bp: 2232
variable_sites: 338
pi: 0.161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb_p1	AAAAAGTGTGCTGTCGACCT	GCCAGTAACCGAGTTGTAGG	45.0	55.0	58.0	58.0	2686 bp	0.5	59.6
rpoBb_p2	TTGATCCCACCACTTTCTGC	GCCAGTAACCGAGTTGTAGG	50.0	55.0	58.1	58.0	2718 bp	0.5	59.2
rpoBb_p3	AAAAAGTGTGCTGTCGACCT	TTTTTAACCAGCCAGCAGGT	45.0	45.0	58.0	57.9	2707 bp	0.5	59.0
rpoBb_p4	AAAAAGTGTGCTGTCGACCT	CCGATTTGGCTCTTCTTGGA	45.0	50.0	58.0	57.9	2831 bp	0.5	58.8
rpoBb_p5	AAAAAGTGTGCTGTCGACCT	TCCGATTTGGCTCTTCTTGG	45.0	50.0	58.0	57.9	2832 bp	0.5	58.8