Hotspot

rpoA

Genome · 85427-87193 · 1767 bp

Back to Chaetophoropsis

Pi 0.139

MarkerSeek score 82.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1767
variable_sites: 234
pi: 0.139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGCTTGTTCTGGGAAAACGT	TTCGAAAAGCGTCGTAACCA	45.0	45.0	57.9	58.1	2864 bp	0.5	58.4
rpoA_p2	TTTCCAAGAAGAGCTGGCTG	TTCGAAAAGCGTCGTAACCA	50.0	45.0	58.1	58.1	2822 bp	0.5	58.0
rpoA_p3	GGCTTTCGCGGAAAATCTTC	TTCGAAAAGCGTCGTAACCA	50.0	45.0	58.1	58.1	2887 bp	0.5	58.0
rpoA_p4	GATGGCTGGGAAAACTACGT	TTCGAAAAGCGTCGTAACCA	50.0	45.0	57.9	58.1	2650 bp	0.5	57.9
rpoA_p5	AGCTTGTTCTGGGAAAACGT	AAAAGCGTCGTAACCAGGAG	45.0	50.0	57.9	58.2	2860 bp	0.5	57.9