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atpE-rpoC2

Genome · 94357-94989 · 633 bp

Back to Chaetophoropsis

Pi 0.347

MarkerSeek score 81.10

Primer available yes

Label atpE-rpoC2

Feature Metadata

label_name: atpE-rpoC2
feature_type: igs
region: Genome
length_bp: 633
variable_sites: 182
pi: 0.347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-rpoC2_p1	AATCCGCTTCTGCTTGTTCA	CGAGCAGTTACTAGAAGCCC	45.0	55.0	58.1	57.8	1484 bp	0.5	57.5
atpE-rpoC2_p2	AATCCGCTTCTGCTTGTTCA	GAGCAGTTACTAGAAGCCCG	45.0	55.0	58.1	57.8	1483 bp	0.5	57.5
atpE-rpoC2_p3	TAATCTCCGCTGACTGTCCA	CGAGCAGTTACTAGAAGCCC	50.0	55.0	58.2	57.8	1265 bp	0.5	57.1
atpE-rpoC2_p4	TAATCTCCGCTGACTGTCCA	GAGCAGTTACTAGAAGCCCG	50.0	55.0	58.2	57.8	1264 bp	0.5	57.1
atpE-rpoC2_p5	GGGAAAACTTACGGACTGGG	GAGCAGTTACTAGAAGCCCG	55.0	55.0	58.2	57.8	1912 bp	0.5	56.8