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rpl32-rpoBb

Genome · 90327-91022 · 696 bp

Back to Chaetophoropsis

Pi 0.351

MarkerSeek score 84.30

Primer available yes

Label rpl32-rpoBb

Feature Metadata

label_name: rpl32-rpoBb
feature_type: igs
region: Genome
length_bp: 696
variable_sites: 231
pi: 0.351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-rpoBb_p1	AGACGCACAAGGGCTTAAAA	TATTTGATGGTCGCACTGGG	45.0	50.0	58.0	58.0	1771 bp	0.5	59.5
rpl32-rpoBb_p2	TTTTAGACGCACAAGGGCTT	TATTTGATGGTCGCACTGGG	45.0	50.0	58.0	58.0	1775 bp	0.5	59.5
rpl32-rpoBb_p3	TTTTTAGACGCACAAGGGCT	TATTTGATGGTCGCACTGGG	45.0	50.0	58.0	58.0	1776 bp	0.5	59.5
rpl32-rpoBb_p4	AGACGCACAAGGGCTTAAAA	ATCAACCCATTACGACTGGC	45.0	50.0	58.0	58.0	1741 bp	0.5	59.5
rpl32-rpoBb_p5	TTTTAGACGCACAAGGGCTT	ATCAACCCATTACGACTGGC	45.0	50.0	58.0	58.0	1745 bp	0.5	59.5