Hotspot

rps18-ycf4

Genome · 82254-83712 · 1459 bp

Back to Chaetophoropsis

Pi 0.419

MarkerSeek score 81.10

Primer available yes

Label rps18-ycf4

Feature Metadata

label_name: rps18-ycf4
feature_type: igs
region: Genome
length_bp: 1459
variable_sites: 517
pi: 0.419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-ycf4_p1	TTTTGGTGGAATTACCGGCA	CAAGCTGCTGAACTTGCAAA	45.0	45.0	58.0	57.8	1651 bp	0.5	58.3
rps18-ycf4_p2	TTTTGGTGGAATTACCGGCA	AAACAAGCTGCTGAACTTGC	45.0	45.0	58.0	57.8	1654 bp	0.5	58.3
rps18-ycf4_p3	TTTTGGTGGAATTACCGGCA	TTTTTCGCTGGGGTTTTCCT	45.0	45.0	58.0	58.2	1845 bp	0.5	58.2
rps18-ycf4_p4	TTTTGGTGGAATTACCGGCA	CAACCCTCAACGTACCCTTT	45.0	50.0	58.0	57.7	1748 bp	0.5	57.8
rps18-ycf4_p5	CGTTAGTTTTCCCAGCCACT	CAAGCTGCTGAACTTGCAAA	50.0	45.0	58.1	57.8	2188 bp	0.5	57.4