Hotspot

rpl5-rps7

Genome · 68186-68784 · 599 bp

Back to Chaetophoropsis

Pi 0.418

MarkerSeek score 82.30

Primer available yes

Label rpl5-rps7

Feature Metadata

label_name: rpl5-rps7
feature_type: igs
region: Genome
length_bp: 599
variable_sites: 225
pi: 0.418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl5-rps7_p1	GCTTCACCTACACCACGATT	GTGCTAGAGCTATCGCCATC	50.0	55.0	57.9	58.0	969 bp	0.5	59.2
rpl5-rps7_p2	GCTTCACCTACACCACGATT	TTGAAGTCAAACCACGTCGT	50.0	45.0	57.9	58.0	1038 bp	0.5	59.1
rpl5-rps7_p3	GCTTCACCTACACCACGATT	TATGGCTCGTAAAGGTGCAC	50.0	50.0	57.9	58.0	1256 bp	0.5	59.1
rpl5-rps7_p4	GCTTCACCTACACCACGATT	TGAATTCACGTCGTCCAGAC	50.0	50.0	57.9	57.9	804 bp	0.5	58.8
rpl5-rps7_p5	GCTTCACCTACACCACGATT	CGTGTTGAAGTCAAACCACG	50.0	50.0	57.9	57.9	1042 bp	0.5	58.6