Hotspot

atpB-rpl5

Genome · 66506-67642 · 1137 bp

Back to Chaetophoropsis

Pi 0.37

MarkerSeek score 82.10

Primer available yes

Label atpB-rpl5

Feature Metadata

label_name: atpB-rpl5
feature_type: igs
region: Genome
length_bp: 1137
variable_sites: 372
pi: 0.37
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rpl5_p1	ACATGCTTTTACCCCTTGCA	AACGTTTTCCCAGAACAGCT	45.0	45.0	58.0	57.9	2075 bp	0.5	59.5
atpB-rpl5_p2	AGACTGTAGGACTTGCACCT	AACGTTTTCCCAGAACAGCT	50.0	45.0	58.0	57.9	1971 bp	0.5	59.4
atpB-rpl5_p3	GCATGATCCCACGATTCACT	AACGTTTTCCCAGAACAGCT	50.0	45.0	58.0	57.9	2199 bp	0.5	59.3
atpB-rpl5_p4	CTGCATGATCCCACGATTCA	AACGTTTTCCCAGAACAGCT	50.0	45.0	58.0	57.9	2201 bp	0.5	59.2
atpB-rpl5_p5	ACTGAATCTCCCTTCCCGAA	AACGTTTTCCCAGAACAGCT	50.0	45.0	58.0	57.9	1920 bp	0.5	59.2