Hotspot

psaJ-rpl14

Genome · 58581-58772 · 192 bp

Back to Chaetophoropsis

Pi 0.225

MarkerSeek score 80.20

Primer available yes

Label psaJ-rpl14

Feature Metadata

label_name: psaJ-rpl14
feature_type: igs
region: Genome
length_bp: 192
variable_sites: 43
pi: 0.225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl14_p1	AGCAAAAGCAACAACTGGTG	ATTTTTGGTCCTGTAGCCCG	45.0	50.0	57.7	58.2	321-568 bp	1.0	84.2
psaJ-rpl14_p2	AAGCAAAAGCAACAACTGGT	ATTTTTGGTCCTGTAGCCCG	40.0	50.0	56.9	58.2	322-569 bp	1.0	78.1
psaJ-rpl14_p3	ACCAAGCAAAAGCAACAACT	ATTTTTGGTCCTGTAGCCCG	40.0	50.0	56.9	58.2	325-572 bp	1.0	78.1
psaJ-rpl14_p4	AGCAAAAGCAACAACTGGTG	GTCCTGTAGCCCGTGAATTA	45.0	50.0	57.7	57.0	314-561 bp	1.0	77.9
psaJ-rpl14_p5	AGCAAAAGCAACAACTGGTG	TCCTGTAGCCCGTGAATTAC	45.0	50.0	57.7	57.0	313-560 bp	1.0	77.9