Hotspot

ycf89

IRa · 60749-61774 · 1026 bp

Back to Chaetoceros

Pi 0.159

MarkerSeek score 82.10

Primer available yes

Label ycf89

Feature Metadata

label_name: ycf89
feature_type: gene
region: IRa
length_bp: 1026
variable_sites: 375
pi: 0.159
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89_p1	AAAATGTTGGCCCAACTTGC	CATTGCGGAAAATTCCCCAC	45.0	50.0	58.0	58.0	2153 bp	0.111111	44.1
ycf89_p2	AAAATGTTGGCCCAACTTGC	TCTCGTACGACTTGCATGTG	45.0	50.0	58.0	58.0	1877 bp	0.111111	44.0
ycf89_p3	AAAATGTTGGCCCAACTTGC	TATTGCTCCGTCAGGCTTTC	45.0	50.0	58.0	58.0	2176 bp	0.111111	44.0
ycf89_p4	AAAATGTTGGCCCAACTTGC	AGTTGACTTGTCCAACCACC	45.0	50.0	58.0	57.9	2359 bp	0.111111	43.7
ycf89_p5	AAAATGTTGGCCCAACTTGC	GTTGACTTGTCCAACCACCT	45.0	50.0	58.0	57.9	2358 bp	0.111111	43.7
ycf89_p1	CATTGCGGAAAATTCCCCAC	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.0	58.1	2299 bp	0.111111	44.0
ycf89_p2	TCTCGTACGACTTGCATGTG	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.0	58.1	2023 bp	0.111111	43.8
ycf89_p3	TATTGCTCCGTCAGGCTTTC	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.0	58.1	2322 bp	0.111111	43.8
ycf89_p4	AGTTGACTTGTCCAACCACC	TGACGTTGTCCCAACTTCTG	50.0	50.0	57.9	58.1	2505 bp	0.111111	43.6
ycf89_p5	GTTGACTTGTCCAACCACCT	TGACGTTGTCCCAACTTCTG	50.0	50.0	57.9	58.1	2504 bp	0.111111	43.6