Hotspot

ycf41

LSC · 99813-100133 · 321 bp

Back to Chaetoceros

Pi 0.317

MarkerSeek score 64.30

Primer available yes

Label ycf41

Feature Metadata

label_name: ycf41
feature_type: gene
region: LSC
length_bp: 321
variable_sites: 181
pi: 0.317
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf41_p1	CGATTTGGATTTCGCGATGG	GGTTTCTTTGAGTGGGGTCA	50.0	50.0	58.0	57.6	834-860 bp	0.333333	54.3
ycf41_p2	CGATTTGGATTTCGCGATGG	TGGTTTCTTTGAGTGGGGTC	50.0	50.0	58.0	57.6	835-861 bp	0.333333	54.3
ycf41_p3	CGATTTGGATTTCGCGATGG	AATTAGCCGGTCAAACTGCT	50.0	45.0	58.0	57.8	931 bp	0.111111	42.7
ycf41_p4	CGATTTGGATTTCGCGATGG	CAATTAGCCGGTCAAACTGC	50.0	50.0	58.0	57.7	932 bp	0.111111	42.2
ycf41_p5	GGATTTCGCGATGGATCACT	AATTAGCCGGTCAAACTGCT	50.0	45.0	58.1	57.8	925 bp	0.111111	41.8