Hotspot

syfB

SSC · 17531-19645 · 2115 bp

Back to Chaetoceros

Pi 0.253

MarkerSeek score 78.10

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 1122
pi: 0.253
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	GCTGACCTGGACGATCTTTT	ACTTTGGAAACTCGCGATGT	50.0	45.0	57.9	58.1	2866 bp	0.111111	42.7
syfB_p2	AGTGAAGATTTGGGCGAGTG	ACTTTGGAAACTCGCGATGT	50.0	45.0	58.2	58.1	2713 bp	0.111111	42.0
syfB_p3	GCTGACCTGGACGATCTTTT	AGGTACTTTGGAAACTCGCG	50.0	50.0	57.9	58.2	2870 bp	0.111111	42.0
syfB_p4	GTGAAGATTTGGGCGAGTGA	ACTTTGGAAACTCGCGATGT	50.0	45.0	58.2	58.1	2712 bp	0.111111	41.9
syfB_p5	TGAAGATTTGGGCGAGTGAC	ACTTTGGAAACTCGCGATGT	50.0	45.0	58.2	58.1	2711 bp	0.111111	41.9