Hotspot

rpoC2

LSC · 67029-71288 · 4260 bp

Back to Chaetoceros

Pi 0.201

MarkerSeek score 65.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4260
variable_sites: 1552
pi: 0.201
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACACAATGATGGCCGTTCAA	GTCGGGCCAAGTATACCAAG	45.0	55.0	58.4	58.1	823 bp	0.111111	41.0
rpoC2_p2	TTGACACAATGATGGCCGTT	GTCGGGCCAAGTATACCAAG	45.0	55.0	58.4	58.1	826 bp	0.111111	41.0
rpoC2_p3	ACACAATGATGGCCGTTCAA	CTATGGATTTTGTCGGGCCA	45.0	50.0	58.4	57.9	834 bp	0.111111	41.0
rpoC2_p4	TTGACACAATGATGGCCGTT	CTATGGATTTTGTCGGGCCA	45.0	50.0	58.4	57.9	837 bp	0.111111	41.0
rpoC2_p5	ACACAATGATGGCCGTTCAA	ACTATGGATTTTGTCGGGCC	45.0	50.0	58.4	57.9	835 bp	0.111111	40.9