Hotspot

ycf89-trnP(ugg)

IRa · 61775-62072 · 298 bp

Back to Chaetoceros

Pi 0.283

MarkerSeek score 72.20

Primer available yes

Label ycf89-trnP(ugg)

Feature Metadata

label_name: ycf89-trnP(ugg)
feature_type: igs
region: IRa
length_bp: 298
variable_sites: 178
pi: 0.283
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-trnP(ugg)_p1	TTAATGTTACAGGAGCGGCC	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.0	58.1	963 bp	0.111111	43.8
ycf89-trnP(ugg)_p2	AGTCTACCATGGACTGCTGT	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.1	58.1	900 bp	0.111111	43.6
ycf89-trnP(ugg)_p3	TTAATGTTACAGGAGCGGCC	GACGTTGTCCCAACTTCTGA	50.0	50.0	58.0	57.8	962 bp	0.111111	42.3
ycf89-trnP(ugg)_p4	AGTCTACCATGGACTGCTGT	GACGTTGTCCCAACTTCTGA	50.0	50.0	58.1	57.8	899 bp	0.111111	42.1
ycf89-trnP(ugg)_p5	TTACCAGGCTGTCCTTCACT	TGACGTTGTCCCAACTTCTG	50.0	50.0	58.3	58.1	1435 bp	0.111111	41.9