Hotspot

trnI(gau)-rns

IRa · 59013-59077 · 65 bp

Back to Chaetoceros

Pi 0.164

MarkerSeek score 80.40

Primer available yes

Label trnI(gau)-rns

Feature Metadata

label_name: trnI(gau)-rns
feature_type: igs
region: IRa
length_bp: 65
variable_sites: 24
pi: 0.164
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(gau)-rns_p1	CTGGGCCATTCTGGATTTGA	GACTGGGGTGAAGTCGTAAC	50.0	55.0	57.9	57.9	199-202 bp	1.0	87.0
trnI(gau)-rns_p2	CTGGGCCATTCTGGATTTGA	GGGGTGAAGTCGTAACAAGG	50.0	55.0	57.9	58.2	195-198 bp	1.0	86.1
trnI(gau)-rns_p3	CTGGGCCATTCTGGATTTGA	GTAACCGTACTGGAAGGTGC	50.0	55.0	57.9	58.3	176-179 bp	1.0	85.5
trnI(gau)-rns_p4	CTGGGCCATTCTGGATTTGA	TGACTGGGGTGAAGTCGTAA	50.0	50.0	57.9	58.3	200-203 bp	1.0	85.3
trnI(gau)-rns_p5	CTGGGCCATTCTGGATTTGA	AGGTAACCGTACTGGAAGGT	50.0	50.0	57.9	57.7	178-181 bp	1.0	85.2