Hotspot

ycf1

SSC · 124204-129693 · 5490 bp

Back to Chaerophyllum

Pi 0.0211

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5490
variable_sites: 173
pi: 0.0211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTGGTTAGTTTCGCCGGA	AGAGTTTTAAAAGAAGGAACAAA	45.0	26.1	58.0	52.7	179 bp	1.0	46.3
ycf1_p2	TTTGGTTAGTTTCGCCGGAT	AAGAGTTTTAAAAGAAGGAACAA	45.0	26.1	57.8	52.7	179 bp	1.0	46.3
ycf1_p3	TTTGGTTAGTTTCGCCGGAT	AGAGTTTTAAAAGAAGGAACAAA	45.0	26.1	57.8	52.7	178 bp	1.0	46.3
ycf1_p4	TTTTGGTTAGTTTCGCCGGA	AGAGTTTTAAAAGAAGGAACAAAA	45.0	25.0	58.0	53.5	179 bp	1.0	46.3
ycf1_p5	TTTTGGTTAGTTTCGCCGGA	AAGAGTTTTAAAAGAAGGAACAA	45.0	26.1	58.0	52.7	180 bp	1.0	46.2