Hotspot

psbH-petB

LSC · 74980-75102 · 123 bp

Back to Chaerophyllum

Pi 0.0271

MarkerSeek score 66.90

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 5
pi: 0.0271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	AACAGCTCCTTTGATGGGTG	AGATGACAGACCCGTTCTCT	50.0	50.0	58.1	57.8	293-294 bp	1.0	78.9
psbH-petB_p2	AACAGCTCCTTTGATGGGTG	TGACAGACCCGTTCTCTGTA	50.0	50.0	58.1	57.7	290-291 bp	1.0	78.4
psbH-petB_p3	AACAGCTCCTTTGATGGGTG	GATGACAGACCCGTTCTCTG	50.0	55.0	58.1	57.7	292-293 bp	1.0	78.4
psbH-petB_p4	AACAGCTCCTTTGATGGGTG	ACAGACCCGTTCTCTGTACT	50.0	50.0	58.1	57.7	288-289 bp	1.0	78.4
psbH-petB_p5	GAACAGCTCCTTTGATGGGT	AGATGACAGACCCGTTCTCT	50.0	50.0	57.8	57.8	294-295 bp	1.0	77.9