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psbT-psbN

LSC · 74433-74523 · 91 bp

Back to Chaerophyllum

Pi 0.0147

MarkerSeek score 66.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 2
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACGATCGAATCTATGGAAGCA	AGAGATCCGTTCGAGGAACA	42.9	50.0	57.0	58.2	231-235 bp	1.0	65.0
psbT-psbN_p2	ACGATCGAATCTATGGAAGCA	TCGAGGAACATGGGGACTAG	42.9	55.0	57.0	58.2	221-225 bp	1.0	64.6
psbT-psbN_p3	ACGATCGAATCTATGGAAGCA	TTCGAGGAACATGGGGACTA	42.9	50.0	57.0	57.8	222-226 bp	1.0	64.5
psbT-psbN_p4	ACCACGATCGAATCTATGGA	AGAGATCCGTTCGAGGAACA	45.0	50.0	55.8	58.2	234-238 bp	1.0	63.8
psbT-psbN_p5	ACGATCGAATCTATGGAAGCA	GGGTACGCCTTATATACCGC	42.9	55.0	57.0	57.7	276-280 bp	1.0	63.7