Hotspot

ycf1

IRb · 107221-107979 · 759 bp

Back to Chaenorhinum

Pi 0.00264

MarkerSeek score 60.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 759
variable_sites: 2
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTGTCATTGACATAACAGATGTC	ATATAGGGGGTGGTCGCATT	41.7	50.0	57.9	58.3	970 bp	1.0	45.2
ycf1_p2	GGTGTCATTGACATAACAGATGTC	GGTGGTCGCATTTCGTCTTA	41.7	50.0	57.9	58.3	962 bp	1.0	45.0
ycf1_p3	GGTGTCATTGACATAACAGATGT	ATATAGGGGGTGGTCGCATT	39.1	50.0	56.9	58.3	970 bp	1.0	45.0
ycf1_p4	GGTGTCATTGACATAACAGATGT	GGTGGTCGCATTTCGTCTTA	39.1	50.0	56.9	58.3	962 bp	1.0	44.8
ycf1_p5	GGTGTCATTGACATAACAGATGTC	TATAGGGGGTGGTCGCATTT	41.7	50.0	57.9	58.5	969 bp	1.0	43.4