Hotspot

rpl20-rps12

LSC · 67643-68472 · 830 bp

Back to Chaenorhinum

Pi 0.00121
MarkerSeek score 59.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
830
variable_sites
1
pi
0.00121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 946-949 bp 1.0 73.6
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 945-948 bp 1.0 73.6
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA AGCGTCGAGGAACATGTACT 50.0 50.0 57.9 58.8 945-948 bp 1.0 72.8
rpl20-rps12_p4 TTGAATGAGCCCCTCGAAAG AGCGTCGAGGAACATGTACT 50.0 50.0 57.9 58.8 944-947 bp 1.0 72.8
rpl20-rps12_p5 TTGAATGAGCCCCTCGAAAG TCAGCGTCGAGGAACATGTA 50.0 50.0 57.9 58.8 946-949 bp 1.0 72.8