Hotspot

psbF-psbE

LSC · 63455-63468 · 14 bp

Back to Chaenorhinum

Pi 0.0714
MarkerSeek score 83.90
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
14
variable_sites
1
pi
0.0714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 CATTGCGGATATTGACCCCA GAGCCGACAAGGAATTCCAT 50.0 50.0 58.0 58.0 195 bp 1.0 79.9
psbF-psbE_p2 CATTGCGGATATTGACCCCA TACAGAGAGCCGACAAGGAA 50.0 50.0 58.0 58.1 201 bp 1.0 79.6
psbF-psbE_p3 CATTGCGGATATTGACCCCA TTACAGAGAGCCGACAAGGA 50.0 50.0 58.0 58.1 202 bp 1.0 79.6
psbF-psbE_p4 GAACAGCCAACCATCGTACT GAGCCGACAAGGAATTCCAT 50.0 50.0 57.9 58.0 145 bp 1.0 79.4
psbF-psbE_p5 GAACAGCCAACCATCGTACT TACAGAGAGCCGACAAGGAA 50.0 50.0 57.9 58.1 151 bp 1.0 79.1