Hotspot

petA-psbJ

LSC · 61913-62938 · 1026 bp

Back to Chaenorhinum

Pi 0.00293

MarkerSeek score 60.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1026
variable_sites: 3
pi: 0.00293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	1134-1135 bp	1.0	76.4
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	1133-1134 bp	1.0	75.9
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1082-1083 bp	1.0	74.9
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1081-1082 bp	1.0	74.5
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1081-1082 bp	1.0	74.1