Hotspot

ycf4-cemA

LSC · 59536-60147 · 612 bp

Back to Chaenorhinum

Pi 0.00327

MarkerSeek score 60.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 2
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.1	58.1	783 bp	1.0	78.9
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	713 bp	1.0	78.7
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	740 bp	1.0	78.4
ycf4-cemA_p4	TAGAGGTCGGGGAGCTATTC	GAGAAATCCACCAGGGCAAA	55.0	50.0	57.7	58.1	803 bp	1.0	77.3
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	716 bp	1.0	77.0