Hotspot

accD-psaI

LSC · 58076-58418 · 343 bp

Back to Chaenorhinum

Pi 0.00583

MarkerSeek score 56.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 343
variable_sites: 2
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	TTGCAAGTGCCGGAAATACT	40.0	45.0	55.5	58.1	504-521 bp	1.0	59.8
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	TTGCAAGTGCCGGAAATACT	40.0	45.0	55.5	58.1	503-520 bp	1.0	59.8
accD-psaI_p3	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCAAGTGC	40.0	45.0	55.5	57.8	514-531 bp	1.0	59.3
accD-psaI_p4	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCAAGTGC	40.0	45.0	55.5	57.8	513-530 bp	1.0	59.3
accD-psaI_p5	AAAAAGCGTTCTGAGTGAGTT	TTGCAAGTGCCGGAAATACT	38.1	45.0	56.3	58.1	504-521 bp	1.0	58.1