Hotspot

psaA-ycf3

LSC · 40736-41464 · 729 bp

Back to Chaenorhinum

Pi 0

MarkerSeek score 59.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	897-906 bp	1.0	79.7
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	917-926 bp	1.0	79.7
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	879-888 bp	1.0	79.7
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	899-908 bp	1.0	79.7
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	826-835 bp	1.0	78.8