Hotspot

atpH-atpI

LSC · 13382-14382 · 1001 bp

Back to Chaenorhinum

Pi 0.001
MarkerSeek score 59.40
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1001
variable_sites
1
pi
0.001
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.1 57.9 1086 bp 1.0 78.3
atpH-atpI_p2 TACCCTCTACAGCTTGACCC TTTTGCAACTTTAGCCGCAG 55.0 45.0 58.1 57.9 1171 bp 1.0 78.0
atpH-atpI_p3 GCGATACCCTCTACAGCTTG TTTTGCAACTTTAGCCGCAG 55.0 45.0 57.9 57.9 1175 bp 1.0 77.9
atpH-atpI_p4 AGCCAATCCAGCAGCAATAA TTTTGCAACTTTAGCCGCAG 45.0 45.0 57.9 57.9 1104 bp 1.0 77.9
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TTTTGCAACTTTAGCCGCAG 50.0 45.0 57.8 57.9 1103 bp 1.0 77.4