Hotspot

trnS-GCU-trnG-UCC

LSC · 8161-8831 · 671 bp

Back to Chaenorhinum

Pi 0.00149
MarkerSeek score 59.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
671
variable_sites
1
pi
0.00149
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 726 bp 1.0 61.2
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 725 bp 1.0 61.2
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 780 bp 1.0 60.1
trnS-GCU-trnG-UCC_p4 AGCAATCCGACGCTTTAGTC ACCACTAAACTATACCCGCT 50.0 45.0 58.4 55.6 728 bp 1.0 58.4
trnS-GCU-trnG-UCC_p5 CGACGCTTTAGTCCACTCAG ACCACTAAACTATACCCGCT 55.0 45.0 58.4 55.6 721 bp 1.0 58.2