Hotspot

psbK-psbI

LSC · 7456-7841 · 386 bp

Back to Chaenorhinum

Pi 0.0104

MarkerSeek score 60.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 4
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	CGCCCTGGATCATTGGATAG	45.0	55.0	58.1	57.9	510 bp	1.0	79.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CGCCCTGGATCATTGGATAG	50.0	55.0	57.9	57.9	505 bp	1.0	79.3
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	ATTACGCCCTGGATCATTGG	45.0	50.0	58.1	57.7	514 bp	1.0	78.0
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	ATTACGCCCTGGATCATTGG	50.0	50.0	57.9	57.7	509 bp	1.0	78.0
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	CGCCCTGGATCATTGGATAG	45.0	55.0	58.3	57.9	502 bp	1.0	77.3